TitleAdipose-derived mesenchymal stem cells enhance healing of mandibular defects in the ramus of swine.
Publication TypeJournal Article
Year of Publication2012
AuthorsWilson, SM, Goldwasser, MS, Clark, SG, Monaco, E, Bionaz, M, Hurley, WL, Rodriguez-Zas, S, Feng, L, Dymon, Z, Wheeler, MB
JournalJ Oral Maxillofac Surg
Date Published2012 Mar
KeywordsAdipose Tissue, Animals, Bone Regeneration, Cell Differentiation, Cell Movement, Cells, Cultured, Disease Models, Animal, Injections, Intralesional, Longitudinal Studies, Male, Mandibular Injuries, Mesenchymal Stem Cell Transplantation, Mesenchymal Stromal Cells, Osteogenesis, Reconstructive Surgical Procedures, Sus scrofa

PURPOSE: This study investigated the effect of adipose-derived mesenchymal stem cells (ASCs) injected locally or systemically on the bone regeneration of a 10-mm-diameter cylindrical noncritical-size defect in the ramus of the pig mandible.

MATERIALS AND METHODS: Fifteen Yorkshire pigs, weighing 60 to 80 kg, received bilateral 10-mm-diameter cylindrical surgical defects in each ramus of the mandible. Pigs received 1) a direct injection into the defect of 2.5 million carboxy-fluorescein diacetate succinimidyl ester-labeled ASCs from 1 of 2 pig donors (n = 6); 2) an ear vein injection of 5 million carboxy-fluorescein diacetate succinimidyl ester-labeled ASCs from 1 of 2 pig donors (n = 6); or 3) an ear vein injection of culture Dulbecco's Modified Eagle's Medium without stem cells (control; n = 3). Pigs from each treatment were sacrificed at 1 hour, 2 weeks, or 4 weeks after surgery. Healing of the defect was evaluated by dual-energy x-ray absorptiometry, micro-computed tomography, fluorescent microscopy, and histology.

RESULTS: Bone healing was accelerated in the ASC-injected treatment groups at 2 and 4 weeks after surgery compared with the control pigs.

CONCLUSIONS: Results from this animal model provide evidence that the injection of ASC locally into a bone defect or systemically can accelerate the healing of bone.

Alternate JournalJ. Oral Maxillofac. Surg.
PubMed ID22374062