|Title||De-novo assembly and analysis of the heterozygous triploid genome of the wine spoilage yeast Dekkera bruxellensis AWRI1499.|
|Publication Type||Journal Article|
|Year of Publication||2012|
|Authors||Curtin, CD, Borneman, AR, Chambers, PJ, Pretorius, IS|
|Keywords||Dekkera, Genome, Fungal, Haplotypes, Open Reading Frames, Phylogeny, Polymorphism, Single Nucleotide, Sequence Analysis, DNA, Wine|
Despite its industrial importance, the yeast species Dekkera (Brettanomyces) bruxellensis has remained poorly understood at the genetic level. In this study we describe whole genome sequencing and analysis for a prevalent wine spoilage strain, AWRI1499. The 12.7 Mb assembly, consisting of 324 contigs in 99 scaffolds (super-contigs) at 26-fold coverage, exhibits a relatively high density of single nucleotide polymorphisms (SNPs). Haplotype sampling for 1.2% of open reading frames suggested that the D. bruxellensis AWRI1499 genome is comprised of a moderately heterozygous diploid genome, in combination with a divergent haploid genome. Gene content analysis revealed enrichment in membrane proteins, particularly transporters, along with oxidoreductase enzymes. Availability of this assembly and annotation provides a resource for further investigation of genomic organization in this species, and functional characterization of genes that may confer important phenotypic traits.
|Alternate Journal||PLoS ONE|
|PubMed Central ID||PMC3314683|